- 斑點(diǎn)雜交
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dot blot hybridization中文什么意思
例句與用法
- Bl21 . the dot blot hybridization verified that the scfv was secreted to the medium
從培養(yǎng)基中可檢測(cè)到可溶性抗人絨毛膜促性腺激素的單鏈抗體。 - Lastly by using the technique of dot blot hybridization , the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer . the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe . by comparing the diagnostic ways of nucleotide probe and fc , the technique of nucleotide probe were proved to have high sensitivity and speci fi city
最后，用地高辛隨機(jī)引物法標(biāo)記成momp基因核酸探針，斑點(diǎn)雜交檢測(cè)衣原體基因組dna ，靈敏度可達(dá)10pg ，且不能檢出其它病原體的核酸。將核酸探針?lè)ㄅc補(bǔ)體結(jié)合反應(yīng)法對(duì)衣原體感染的診斷進(jìn)行比較，初步證明該探針具有較高的敏感性與較強(qiáng)的特異性。 - The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii . two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium . the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome . at the same time ; compared agrabactenum - mediated method with gene gun method , the transformation frequency of the former was 16 . 7 % , while the latter was 50 % , so gene gun transformation method was suitable for long ya liiliwn
用攜帶有幾丁質(zhì)酶基因和- 1 、 3葡聚糖酶基因的工程菌，通過(guò)農(nóng)桿菌介導(dǎo)法和基因槍轉(zhuǎn)化法轉(zhuǎn)化龍牙百合，經(jīng)pcr和點(diǎn)雜交檢測(cè)證明外源基因已經(jīng)整合到植物染色體中。同時(shí)對(duì)農(nóng)桿菌介導(dǎo)法和基因槍法進(jìn)行比較，發(fā)現(xiàn)農(nóng)桿菌介導(dǎo)法的轉(zhuǎn)化率為16 . 7 ，基因槍法的轉(zhuǎn)化率為50 ，因此可能基因槍轉(zhuǎn)化法更適于龍牙百合的遺傳轉(zhuǎn)化。 - Was multiplied and the tk gene was cloned . the cloned tk gene was retrieved by proper restrictive hemodynamics . the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin . then , the specificity and sensitivity of tk gene probe were detected with dot blot hybridization . the sequence of tk gene of nm98a strain was analysed . the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99 . 7 %
本研究中首次對(duì)iltv - nm98a株的tk基因進(jìn)行了克隆和序列分析，結(jié)果表明： iltv - nm98a株tk基因的核苷酸序列與已發(fā)表的iltvtk基因的核苷酸序列具有高度的同源性，兩者之間僅相差4個(gè)核苷酸，同源性高達(dá)99 . 7 ，從而證實(shí)了iltvtk基因是高度保守的，為iltvtk基因核酸探針的制備提供了有力的依據(jù)。